Anti-Insulin Receptor alpha antibody (ab203037)
Key features and details
- Rabbit polyclonal to Insulin Receptor alpha
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Insulin Receptor alpha antibody
See all Insulin Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Insulin Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Insulin Receptor alpha aa 700-800 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P06213 -
Positive control
- IHC-P: Rat tongue, brain, lung and small intestine tissue; WB: Human MCF-7, Jurkat, SW480 ad LOVO cell lysates; Flow Cyt: HL-60 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Insulin Receptor alpha antibody (ab203037)All lanes : Anti-Insulin Receptor alpha antibody (ab203037) at 1/500 dilution
Lane 1 : Human MCF-7 cell lysate
Lane 2 : Human Jurkat cell lysate
Lane 3 : Human SW480 cell lysate
Lane 4 : Human LOVO cell lysate
Secondary
All lanes : Conjugated secondary antibody for 60 minutes at 37C at 1/20000 dilution
Predicted band size: 156 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor antibody (ab203037)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat tongue tissue labeling Insulin Receptor with ab203037 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody (ab203037)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat small intestine tissue labeling Insulin Receptor alpha with ab203037 at 1/200 dilution, followed by conjugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval with sodium citrate buffer, pH 6 was performed for 15 minutes.
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Flow Cytometry - Anti-Insulin Receptor alpha antibody (ab203037)Flow cytometric analysis of HL-60 cells labelleing insulin receptor alpha with ab203037 at 1/100 dilution (green), compared to control cells (blue) and isotype control (orange).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody (ab203037)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat small brain tissue labeling Insulin Receptor alpha with ab203037 at 1/200 dilution, followed by conjugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval with sodium citrate buffer, pH 6 was performed for 15 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody (ab203037)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Insulin Receptor alpha with ab203037 at 1/200 dilution, followed by conjugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval with sodium citrate buffer, pH 6 was performed for 15 minutes.
