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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] (ab62321)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] (ab62321)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP351(2)Y] to Insulin Receptor (phospho Y1185)
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y]
    See all Insulin Receptor primary antibodies
  • Description

    Rabbit monoclonal [EP351(2)Y] to Insulin Receptor (phospho Y1185)
  • Host species

    Rabbit
  • Specificity

    ab62321 detects Insulin Receptor when phosphorylated at Tyrosine 1185.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 1185 of the human Insulin Receptor.

  • Positive control

    • HepG2 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP351(2)Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cancer
    • Growth factors
    • Insulin and insulin-like
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] (ab62321)
    Western blot - Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] (ab62321)
    All lanes : Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] (ab62321) at 1/500 dilution

    Lane 1 : HepG2 cell lysate; untreated
    Lane 2 : HepG2 cell lysate; Insulin treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution

    Predicted band size: 156 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?



    Beta Tubulin has been included as a loading control.
  • Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] (ab62321)
    Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] (ab62321)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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