Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2871] to Ret - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC/IF, ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ret antibody [EPR2871] - BSA and Azide free
    See all Ret primary antibodies

  • Description

    Rabbit monoclonal [EPR2871] to Ret - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Neuro-2a, SH-SY5Y and TT cell lysates and mouse and rat brain tissue lysates. IHC-P: Human thyroid gland carcinoma, human gastric carcinoma and mouse colon tissues. ICC/IF: Neuro-2a cells. Flow Cyt: Caco-2 cells. IP: Neuro-2a cell lysate.

  • General notes

    Ab214791 is the carrier-free version of ab134100. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab214791 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue labelling Ret with purified ab134100 at a dilution of 1/50. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134100).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)

     

    Immunocytochemistry/Immunofluorescence analysis of TT (human thyroid carcinoma epithelial cell) cells labelling Ret with purified ab134100 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (1/200) was used as a counter stain.

    -ve control: Caco-2 (PMID: 10811228) cells stained with primary antibody (1/100) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134100).

  • Immunoprecipitation - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    Immunoprecipitation - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)

    ab134100 (purified) at 1/40 immunoprecipitating Ret in Neuro-2a whole cell lysate.

    Lane 1 (input): Neuro-2a whole cell lysate (10µg)

    Lane 2 (+): ab134100 + Neuro-2a whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134100 in Neuro-2a whole cell lysate.

    For western blotting, ab134100 was used at 1/1000 followed by a HRP-conjugated anti-rabbit IgG (specific to the non-reduced form of IgG, 1/1500).

    Blocking buffer and dilution concentration: 5% NFDM/TBST.

    We are unsure about the nature of 40kDa to 60kDa bands, they might be the intracellular fragments of Ret.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134100).

  • ELISA - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    ELISA - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    ELISA antigen dose-response curve using purified ab134100 at 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An Alkaline-Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134100).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)

    Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling Ret with purified ab134100 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134100).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Ret with unpurified ab134100 at a dilution of 1/50 followed by HRP-conjugated goat anti-rabbit IgG (H&L) (ab97051, 1/500). Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134100).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling Ret with unpurified ab134100 at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134100).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)
    Anti-Ret antibody [EPR2871] - BSA and Azide free (ab214791)

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