Anti-Ret (phospho Y1015) antibody (ab74154)
Key features and details
- Rabbit polyclonal to Ret (phospho Y1015)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Ret (phospho Y1015) antibody
See all Ret primary antibodies -
Description
Rabbit polyclonal to Ret (phospho Y1015) -
Host species
Rabbit -
Specificity
ab74154 detects endogenous levels of Ret only when phosphorylated at tyrosine 1015 (Human: Tyr1015; Mouse: Tyr1016; Rat: Tyr1037). -
Tested Applications & Species
See all applications and species dataApplication Species WB African green monkey
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Immunogen
Synthetic phosphopeptide derived from human Ret around the phosphorylation site of tyrosine 1015 (R-D-YP-L-D).
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Positive control
- Extracts from COS7 cells treated with EGF (200ng/ml, 30mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74154 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ret (phospho Y1015) antibody (ab74154)All lanes : Anti-Ret (phospho Y1015) antibody (ab74154) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from COS7 cells
treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 124 kDa
Observed band size: 124 kDa
