Anti-Ret antibody - Extracellular domain (ab205557)
Key features and details
- Chicken polyclonal to Ret - Extracellular domain
- Suitable for: ICC/IF, WB
- Reacts with: Human, Chinese hamster
- Isotype: IgY
Overview
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Product name
Anti-Ret antibody - Extracellular domain
See all Ret primary antibodies -
Description
Chicken polyclonal to Ret - Extracellular domain -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Chinese hamster
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Immunogen
Recombinant fragment (His-tag) corresponding to Human Ret (extracellular). Recombinant His-tagged extracellular domain of Human Ret produced in CHO-suspension cell line and then purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Database link: P07949 -
Positive control
- Ret expressed in CHO cells; Human Ret expressed in U2OS cells.
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General notes
As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 99% PBS, Ammonium sulphate
Concentrated ammonium sulphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-Ret antibody - Extracellular domain (ab205557)Anti-Ret antibody - Extracellular domain (ab205557) at 0.2 µg/ml + Ret expressed in CHO cells at 0.1 µg
Secondary
Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
Predicted band size: 124 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody - Extracellular domain (ab205557)Immunofluorescent analysis of Human Ret expressed in U2OS cells labeling Ret with ab205557 at 1/500, Goat anti-chicken IgY - H&L DyLight 550 (ab96952) at 1/200 as secondary antibody and DAPI staining (blue).
