Recombinant mouse EGFR protein (Active) (ab196107)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 38% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse EGFR protein (Active)
See all EGFR proteins and peptides -
Biological activity
Specific Activity: 49 pmole/min/mg
Assay Conditions: Assay was performed in a Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. The reaction to place at 30°C for 40 min. The amount of ATP transferred was calculated using Kinase reagent. -
Purity
>= 38 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKIKV LGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMA SVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYVREHKDNIGSQYLLNW CVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEK EYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDG IPASDISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELILE FSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMEDVVDADEYL IPQQGFFNSPSTSRTPLLSSLSATSNNSTVACINRNGSCRVKEDAFLQRY SSDPTGAVTEDNIDDAFLPVPEYVNQSVPKRPAGSVQNPVYHNQPLHPAP GRDLHYQNPHSNAVGNPEYLNTAQPTCLSSGFNSPALWIQKGSHQMSLDN PDYQQDFFPKETKPNGIFKGPTAENAEYLRVAPPSSEFIGA -
Predicted molecular weight
62 kDa including tags -
Amino acids
670 to 1210 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_207655/NP_997538
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 2.03% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant mouse EGFR protein (Active) (ab196107)
Specific activity of ab196107 was determined to be 49 pmol/min/mg
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SDS-PAGE - Recombinant mouse EGFR protein (Active) (ab196107)10% SDS Page analysis of ab196107 (1 μg)
