Recombinant mouse EGFR protein (ab167752)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 94% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant mouse EGFR protein (ab167752)Captured Mouse EGF R, His Tag on CM5 Chip via anti-His antibody can bind Mouse EGF, Fc Tag with an affinity constant of 5.44 nM as determined in SPR assay (Biacore T200).
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SDS-PAGE - Recombinant mouse EGFR protein (ab167752)Mouse EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. -
Functional Studies - Recombinant mouse EGFR protein (ab167752)Immobilized Mouse EGF, Fc Tag at 2 µg/mL (100 µL/well) can bind Mouse EGF R, His Tag with a linear range of 0.02-0.313 µg/mL. -
SDS-PAGE - Recombinant mouse EGFR protein (ab167752)SDS-PAGE analysis of reduced ab167752 stained overnight with Coomassie Blue.
