Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIE2 antibody [EPR21915] - BSA and Azide free (ab238172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21915] to TIE2 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TIE2 antibody [EPR21915] - BSA and Azide free
    See all TIE2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21915] to TIE2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HUVEC cells.

  • General notes

    Ab238172 is the carrier-free version of ab221154. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238172 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-TIE2 antibody [EPR21915] - BSA and Azide free (ab238172)
    Flow Cytometry - Anti-TIE2 antibody [EPR21915] - BSA and Azide free (ab238172)

    Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (left panel) and HUVEC (human umbilical vein endothelial cell line) (right panel) cells labeling TIE2 with ab221154 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) Isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: HEK-293T (PMID: 17189382)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221154).

  • Immunocytochemistry/ Immunofluorescence - Anti-TIE2 antibody [EPR21915] - BSA and Azide free (ab238172)
    Immunocytochemistry/ Immunofluorescence - Anti-TIE2 antibody [EPR21915] - BSA and Azide free (ab238172)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling TIE2 with ab221154 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in HUVEC cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: HEK-293T (PMID: 17189382).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221154).

  • Anti-TIE2 antibody [EPR21915] - BSA and Azide free (ab238172)
    Anti-TIE2 antibody [EPR21915] - BSA and Azide free (ab238172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TIE2 antibody [EPR21915] - BSA and Azide free (ab238172)

© 2021 Astana Biomed Group LLP. All rights reserved.