Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR (phospho Y845) antibody [EPR2149Y] - BSA and Azide free (ab240934)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2149Y] to EGFR (phospho Y845) - BSA and Azide free
  • Suitable for: ICC/IF, WB, Dot blot, Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR (phospho Y845) antibody [EPR2149Y] - BSA and Azide free
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [EPR2149Y] to EGFR (phospho Y845) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, Dot blot, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: EGF treated A431 cells.

  • General notes

    ab240934 is the carrier-free version of ab97613.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Images

  • Flow Cytometry - Anti-EGFR (phospho Y845) antibody [EPR2149Y] - BSA and Azide free (ab240934)
    Flow Cytometry - Anti-EGFR (phospho Y845) antibody [EPR2149Y] - BSA and Azide free (ab240934)

    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 10 minutes cells labeling EGFR with purified ab97613 at 1/400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). Untreated A431 cells (Green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab97613).

  • Dot Blot - Anti-EGFR antibody [EPR2149Y] - BSA and Azide free (ab240934)
    Dot Blot - Anti-EGFR antibody [EPR2149Y] - BSA and Azide free (ab240934)

    Dot Blot analysis.

    Lane 1: EGFR (pY845) phospho peptide.

    Lane 2: EGFR non-phospho peptide labeling EGFR (phospho Y845) with ab97613 (unpurified) at 1/1000 dilution.

    5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab97613)

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EPR2149Y] - BSA and Azide free (ab240934)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EPR2149Y] - BSA and Azide free (ab240934)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cell lines labeling EGFR with ab97613 (purified) at 1/50 dilution,followed by Goat anti-Rabbit IgG H & L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution

    Cells were counterstained with ab195889 (Anti-alpha Tubulin antibody [DM1A] ) - Microtubule Marker (Alexa Fluor® 594) 

    The nuclear counterstain is DAPI (blue).

    The green staining on the membrane was increased in the EGF (100 ng/ml, 10 min) treated A431 cells when compared with A431 cells without treatment. After LP treatment, the green signaling was obviously decreased.

    For the pan antibody, there was no great difference after EGF (100 ng/ml, 10 min) or EGF (100 ng/ml, 10 min) + LP treatment. The data showed mostly membranous staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab97613)

  • Anti-EGFR (phospho Y845) antibody [EPR2149Y] - BSA and Azide free (ab240934)
    Anti-EGFR (phospho Y845) antibody [EPR2149Y] - BSA and Azide free (ab240934)

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