Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)
Key features and details
- Rabbit polyclonal to IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)
- Suitable for: WB
- Reacts with: Chinese hamster, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody
See all IGF1 Receptor primary antibodies -
Description
Rabbit polyclonal to IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Chinese hamster
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Immunogen
Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor. The corresponding residues in the IGF1R are 1131, 1135 and 1136.
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Positive control
- WB: CHO-T (Chinese hamster ovary cell line) whole cell extract transfected with a vector encoding the human insulin receptor and stimulated with insulin.
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General notes
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR) as numbered according to Ebina, et al. (1146, 1150 and 1151 according to Ullrich, et al.). The corresponding residues in the IGF1R are 1131, 1135 and 1136. The sequence is conserved in human, mouse and rat for both the IR and IGF1R.The two relevant papers are:Ebina, Y., et al. (1985) The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40(4):747-758.Ullrich, A., et al. (1985) Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313(6005):756-761. There are a long and a short isoform of this protein. This is why we are listing 1158, 1162 and 1163 in the name (where these phospho sites in the long isoform) as well as 1146, 1150 and 1151 (for the short isoform).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Insulin Receptor (IR). The final product is generated by affinity chromatography using an IR-derived peptide phosphorylated at tyrosines 1158, 1162 and 1163 (1131, 1135 and 1136 for IGF1R). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)All lanes : Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681) at 1/1000 dilution (2 hours at room temperature in a 3% BSA-TBST buffer)
Lane 1 : Unstimulated (-), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C
Lanes 2-8 : Insulin stimulated (+), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C
Secondary
All lanes : goat F(ab’ 2 anti-rabbit IgG HRP conjugatePeptide Competition - prior primary antibody incubation:
1 and 2 - no peptide,
3 - non-phosphorylated peptide corresponding to the immunogen,
4 - generic phosphotyrosine-containing peptide,
5 to 7 - phosphopeptides corresponding to other IR/IGF1R sites,
8 - phosphopeptide immunogen.
10% SDS-PAGE transferred to PVDF.
Method of detection: Pierce SuperSignal method.
The data show that only the phosphopeptide corresponding to IR/IGF1R [pYpYpY1158/1162/1163] completely blocks the antibody signal, thereby demonstrating the specificty.
