Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR antibody [EMab-134] (ab264540)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [EMab-134] to EGFR
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR antibody [EMab-134]
    See all EGFR primary antibodies

  • Description

    Mouse monoclonal [EMab-134] to EGFR

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 and MDA-MB-468 whole cell lysates. IHC-P: Human oral carcinoma and lung carcinoma tissue; Human skin tissue. Flow Cyt: A431 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-EGFR antibody [EMab-134] (ab264540)
    Western blot - Anti-EGFR antibody [EMab-134] (ab264540)
    All lanes : Anti-EGFR antibody [EMab-134] (ab264540) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 134 kDa
    Observed band size: 130,180 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2: 15 secs; Lane 3: 8 secs.

    The molecular weight observed is in consistent with the literature (PMID: 9090976).
    Negative control: SW620 (PMID: 19789347).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] (ab264540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] (ab264540)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling EGFR with ab264540 at 1/100 dilution followed by a ready to use secondary. Membranous staining on human skin is observed. The section was incubated with ab264540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-EGFR antibody [EMab-134] (ab264540)
    Flow Cytometry - Anti-EGFR antibody [EMab-134] (ab264540)

    Flow cytometric analysis of SW620 (human colorectal adenocarcinoma epithelial cell, Left panel), A431 (human epidermoid carcinoma epithelial cell, Right panel) cells labelling EGFR with ab264540 at 1/100 dilution (1µg) (Red) compared with a mouse monoclonal IgG  (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Negative control: SW620.

    Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] (ab264540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] (ab264540)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling EGFR with ab264540 at 1/100 dilution followed by a ready to use secondary. Membranous staining on human lung carcinoma tissue is observed. The section was incubated with ab264540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] (ab264540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EMab-134] (ab264540)

    Immunohistochemical analysis of paraffin-embedded human oral carcinoma tissue labeling EGFR with ab264540 at 1/100 dilution followed by a ready to use secondary. Membranous staining on human oral carcinoma tissue is observed. The section was incubated with ab264540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-EGFR antibody [EMab-134] (ab264540)
    Anti-EGFR antibody [EMab-134] (ab264540)

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