Anti-TIE2 antibody [EPR23823-109] - BSA and Azide free (ab275749)
![Anti-TIE2 antibody [EPR23823-109] - BSA and Azide free (ab275749)](https://www.abcam.com/ps/products/275/ab275749/Images/ab275749-411879-anti-tie2-antibody-epr23823109-western-blot-vein.jpg "Anti-TIE2 antibody [EPR23823-109] - BSA and Azide free (ab275749)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23823-109] to TIE2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-TIE2 antibody [EPR23823-109] - BSA and Azide free
See all TIE2 primary antibodies -
Description
Rabbit monoclonal [EPR23823-109] to TIE2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC whole cell lysate.
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General notes
ab275749 is the carrier-free version of ab259982. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275749 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23823-109 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIE2 antibody [EPR23823-109] - BSA and Azide free (ab275749)All lanes : Anti-TIE2 antibody [EPR23823-109] (ab259982) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 120,150 kDa why is the actual band size different from the predicted?This data was developed using ab259982, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 30444491, 17965739).
Negative control: HEK-293 (PMID: 20973951, 15851516).
Exposure time: 15 seconds.
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![Anti-TIE2 antibody [EPR23823-109] - BSA and Azide free (ab275749)](https://www.abcam.com/ps/products/275/ab275749/Images/ab275749-2-benefits-of-recombinant-antibodies.png)
Anti-TIE2 antibody [EPR23823-109] - BSA and Azide free (ab275749)
