Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)
![Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163948-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-immunohistochemistry.jpg "Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)")
Key features and details
- Mouse monoclonal [OTI1A3] to Indoleamine 2, 3-dioxygenase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3]
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Mouse monoclonal [OTI1A3] to Indoleamine 2, 3-dioxygenase -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Indoleamine 2, 3-dioxygenase aa 1-403.
Sequence:MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLI ESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVR KVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDV LFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKAL LEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMP PAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLL KEG
Database link: NP_002155 -
Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase cDNA; IHC-P: Human kidney carcinoma, lymph node and lymphoma tissues; ICC/IF: COS7 cells transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI1A3 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.
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![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163950-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-western-blot.jpg)
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787) at 1/4000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 45 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163949-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase, labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163947-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163946-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.
