Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP260] to Indoleamine 2, 3-dioxygenase - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free
    See all Indoleamine 2, 3-dioxygenase primary antibodies

  • Description

    Rabbit monoclonal [SP260] to Indoleamine 2, 3-dioxygenase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cytometry: HeLa cells treated with IFN-gamma. IHC-P: Human tonsil, thymus, colon, stomach adenocarcinoma, pancreatic adenocarcinoma, endometrial adenocarcinoma and ovarian adenocarcinoma tissue.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab245737 is the carrier-free version of ab228468. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245737 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228468).

  • Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) treated with 50 ng/ml IFN-gamma for 16 h cells labeling Indoleamine 2, 3-dioxygenase with purified ab228468 at 1/15(9.4µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228468).

  • Flow Cytometry - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Flow Cytometry - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Flow cytometry analysis of HeLa cells treated with 50ng/ml IFN-gamma for 16hours labeling Indoleamine 2, 3-dioxygenase with purified ab228468 at 1/200 dilution (0.705 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.Untreated cells (Green).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228468).

  • Flow Cytometry - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Flow Cytometry - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Flow Cytometry analysis of IFN-gamma treated HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling Indoleamine 2, 3-dioxygenase with ab228468 1/400 dilution (green) compared to a negative control rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228468).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228468).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Formalin-fixed, paraffin-embedded human colon tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228468).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228468).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228468).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228468).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228468).

  • Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)
    Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - BSA and Azide free (ab245737)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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