Anti-Indoleamine 2, 3-dioxygenase antibody (ab235902)
Key features and details
- Rabbit polyclonal to Indoleamine 2, 3-dioxygenase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Rabbit polyclonal to Indoleamine 2, 3-dioxygenase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Indoleamine 2, 3-dioxygenase aa 1 to the C-terminus.
Database link: P14902 -
Positive control
- WB: HeLa, K562, A549, HEK-293T and U-87 MG whole cell lysates. IHC-P: Human cervical cancer tissue. ICC/IF: HepG2 and HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human cervical cancer tissue stained for Indoleamine 2, 3-dioxygenase using ab235902 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody (ab235902) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 45 kDa
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HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Indoleamine 2, 3-dioxygenase (green) using ab235902 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Anti-Indoleamine 2, 3-dioxygenase antibody (ab235902) at 1/500 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
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4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Indoleamine 2, 3-dioxygenase (green) using ab235902 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).