Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9009(2)] to Metabotropic Glutamate Receptor 3/MGLUR3 - BSA and Azide free
  • Suitable for: ICC, ELISA, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free
    See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9009(2)] to Metabotropic Glutamate Receptor 3/MGLUR3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, ELISA, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain tissue lysate. Fetal brain, Human cerebellum and Human hypothalamus tissue lysates; ICC: Mouse primary neuron cells. Flow cyt: Mouse primary neuron and Neuro-2a cells

  • General notes

    ab249349 is the carrier-free version of ab166608 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249349 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Metabotropic Glutamate Receptor 3

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)

    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human MGLUR3 recombinant protein at 500ng/ml with ab166608. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)

    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)

    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Immunocytochemistry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)

    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution + Mouse brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 99 kDa



    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution + Mouse brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 99 kDa
    Observed band size: 220 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution + fetal brain lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 110& 220 kDa why is the actual band size different from the predicted?



    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 220 kDa why is the actual band size different from the predicted?



    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

     

  • ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)

    This data was developed using ab166608, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human MGLUR3 recombinant protein at 500 ng/mL with ab166608. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)

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