Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22955-101] to Connexin 43 / GJA1 - BSA and Azide free
  • Suitable for: IP, IHC-Fr, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free
    See all Connexin 43 / GJA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22955-101] to Connexin 43 / GJA1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody shows low affinity in detecting cell line samples.

  • Tested applications

    Suitable for: IP, IHC-Fr, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain, mouse heart, rat brain and human brain lysates. IHC-P: Human, mouse and rat cardiac muscle tissues. Mouse testis tissue. IP: Mouse brain lysate.

  • General notes

    Ab259276 is the carrier-free version of ab235585. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab259276 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    All lanes : Anti-Connexin 43 / GJA1 antibody [EPR22955-101] (ab235585) at 1/1000 dilution

    Lane 1 : Huh7( Human hepatocellular carcinoma epithelial cell)whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : C2C12 (Mouse myoblasts myoblast)whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Exposure time: 180 seconds


    Blocking and dilution buffer: 5% NFDM/TBST

    This product has low affinity in cell line samples.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235585).

  • Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

    Connexin 43 / GJA1 was immunoprecipitated from 0.35 mg mouse brain lysate 10µg with ab235585 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235585 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain lysate 10µg.

    Lane 2: ab235585 IP in mouse brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235585 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    High MW band between 75kDa and 100kDa represents Connexin 43 dimers which described in literature. (PMID: 19460776).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235585).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Connexin 43 / GJA1 with ab235585 at 1/1000 dilution (0.507 µg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on rat myocardial intercalated disc is observed. The section was incubated with ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235585).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Connexin 43 / GJA1 with ab235585 at 1/1000 dilution (0.507 µg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Membranous staining on mouse testis is observed. The section was incubated with ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235585).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Connexin 43 / GJA1 with ab235585 at 1/1000 dilution (0.507 µg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on mouse myocardial intercalated disc (PMID: 30228305) is observed. The section was incubated with ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235585).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Connexin 43 / GJA1 with ab235585 at 1/500 dilution (1.01 ug/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on human myocardial intercalated disc. The section was incubated with ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235585).

  • Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

    4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of rat cardiac muscle tissue labeling Connexin 43 / GJA1 using ab235585 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counterstain is DAPI (blue). Positive staining on intercalated discs of rat cardiac muscle (PMID: 18678643) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235585).

  • Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

    4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse cardiac muscle tissue labeling Connexin 43 / GJA1 using ab235585 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counterstain is DAPI (blue). Positive staining on intercalated discs of mouse cardiac muscle (PMID: 18678643) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235585).

  • Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
    Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)

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