Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free
    See all Annexin-7/ANXA7 primary antibodies

  • Description

    Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251259 is the carrier-free version of ab198990 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251259 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Annexin VII This product was previously labelled as Annexin VII

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + U87-MG cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab198990, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab198990, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab198990, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab198990, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    This data was developed using ab198990, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue sections labeling ANXA7 with ab198990 at a 1/200 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    This data was developed using ab198990, the same antibody clone in a different buffer formulation.ANXA7 was immunoprecipitated from U87-MG (human glioblastoma) whole cell extract with ab198990 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198990 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: U87-MG (human glioblastoma) whole cell extract (Input) 10 µg.
    Lane 2: ab198990 IP in U87-MG (human glioblastoma) whole cell extract.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198990 in U87-MG (human glioblastoma) whole cell extract. Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)
    Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

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