Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - BSA and Azide free
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free
See all Annexin-7/ANXA7 primary antibodies
Description

Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab251259 is the carrier-free version of ab198990 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251259 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as Annexin VII This product was previously labelled as Annexin VII
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR16173
Isotype

IgG
Research areas

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + U87-MG cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

This data was developed using ab198990, the same antibody clone in a different buffer formulation.
Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + Jurkat cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

This data was developed using ab198990, the same antibody clone in a different buffer formulation.
Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 53 kDa

This data was developed using ab198990, the same antibody clone in a different buffer formulation.
Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

This data was developed using ab198990, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

This data was developed using ab198990, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue sections labeling ANXA7 with ab198990 at a 1/200 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

This data was developed using ab198990, the same antibody clone in a different buffer formulation.ANXA7 was immunoprecipitated from U87-MG (human glioblastoma) whole cell extract with ab198990 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198990 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: U87-MG (human glioblastoma) whole cell extract (Input) 10 µg.
Lane 2: ab198990 IP in U87-MG (human glioblastoma) whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198990 in U87-MG (human glioblastoma) whole cell extract. Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Annexin-7/ANXA7 antibody [EPR16173] - BSA and Azide free (ab251259)

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