Anti-Connexin 43 / GJA1 antibody (ab230537)
Key features and details
- Rabbit polyclonal to Connexin 43 / GJA1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Connexin 43 / GJA1 antibody
See all Connexin 43 / GJA1 primary antibodies -
Description
Rabbit polyclonal to Connexin 43 / GJA1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Connexin 43/ GJA1 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P17302 -
Positive control
- WB: C6, HeLa and C2C12 whole cell lysate; mouse heart and rat brain tissue lysate. IHC-P: Human heart tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Connexin 43 / GJA1 antibody (ab230537)All lanes : Anti-Connexin 43 / GJA1 antibody (ab230537) at 1/500 dilution
Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Rat brain tissue lysate
Predicted band size: 43 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody (ab230537)Formalin-fixed, paraffin-embedded human heart tissue stained for Connexin 43 / GJA1 using ab230537 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody (ab230537)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Connexin 43 / GJA1 using ab230537 at 1/100 dilution in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
