Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

ab171739 (purified ) at 1/50 dilution (2 µg) immunoprecipitating FABP-1 in Human fetal liver lysate.
Lane 1 (input): Human fetal liver lysate 10 µg
Lane 2 (+): ab171739 & Human fetal liver lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab171739 in Human fetal liver lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

Immunohistochemistry of paraffin-embedded Human urinary bladder carcinoma tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).

Immunohistochemistry of paraffin-embedded Human liver tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).

Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate (lane 1) labeling FABP-1 using ab171739 (unpurified) at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Lane 2: 1X PBS (negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).