Phenylalanine Assay Kit (ab83376)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
Overview
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Product name
Phenylalanine Assay Kit -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 2 µM -
Range
2 µM - 20 µM -
Assay time
0h 30m -
Product overview
Phenylalanine Assay Kit ab83376 provides a quick, simple, accurate method for quantifying phenylalanine in biological samples.
In the phenylalanine assay protocol, phenylalanine is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
Phenylalanine assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20 min
- analyze with a microplate reader -
Notes
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Developer (lyophilized) Red 1 vial Enzyme Mix (lyophilized) Green 1 vial Phenyalanine Standard (1.0 µmol) (lyophilized) Yellow 1 vial Phenylalanine Assay Buffer WM 1 x 25ml Tyrosinase (lyophilized) Blue 1 vial -
Research areas
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Relevance
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences. -
Alternative names
- Phe
Images
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OEM testing Phase VI ; Kits & Assays Initiative Assay Testing Jan/Feb 2015
Phenylalanine measured in cell lysates showing quantities (micromoles) per million of cells tested.
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Functional assay - ab83376 Phenylalanine Assay KitPhenylalanine measured in biofluids showing concentration in micromolar.
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Functional assay - ab83376 Phenylalanine Assay KitPhenylalanine fluorimetric standard curve: mean of duplicates (+/- SD) with background readings subtracted
