Phenylalanine Assay Kit (Colorimetric) (ab241000)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Serum, Tissue Lysate
Overview
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Product name
Phenylalanine Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Serum, Cell Lysate, Tissue Lysate -
Product overview
Phenylalanine Assay Kit (Colorimetric) (ab241000) provides a quick, simple and accurate method for quantifying Phenylalanine in biological samples. In the assay, Phe is metabolized with the simultaneous formation of NADH which reacts with a probe to generate absorbance that can be followed colorimetrically at 450 nm. The assay is linear in the range from 0.2 to 10 nmol, allowing accurate determination of concentrations in the range of 1 - 50 µM Phenylalanine.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Phenylalanine Assay Buffer 1 x 25ml Phenylalanine Developer 1 vial Phenylalanine Enzyme Mix 1 vial Phenylalanine Standard (1 micromol) 1 vial Tyrosinase 1 vial -
Research areas
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Relevance
Phenylalanine is the most commonly found aromatic, essential amino acid. In a normal individual it is converted to tyrosine, which in turn is used to synthesise dopamine and norepinephrine (nerurotransmitters). Phenylalanine takes three differnt forms; L-, D- and DL-. The L- form is the most common and the type in which it is incorporated into the body's proteins. The D- form acts as a painkiller and the DL- a combination of the two. Phenylalanine is used to elevate mood as it is involved with the nervous system, it can also aid memory and learning and can been used as an appetite suppressant. Treatment with phenylalanine can be benificial in sufferes of Parkinson's disease, to treat depression and the D form may also be helpful in the treatment of chronic pain in both osteo-arthritis and rheumatoid arthritis. -
Alternative names
- F
- LPA
- Phe
Images
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Phenylalanine Standard Curve
In this instance, standard curve is shown before background subtraction.
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Determination in liver lysate and serum:Liver tissue from rat and human serum were prepared according to the protocol. For Phe determination, 10 µL liver lysate (1.5 mg/ml) and 80 µL tyrosinase-treated serum were used.
