Anti-RIG-I/DDX58 antibody (ab238254)
Key features and details
- Goat polyclonal to RIG-I/DDX58
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RIG-I/DDX58 antibody
See all RIG-I/DDX58 primary antibodies -
Description
Goat polyclonal to RIG-I/DDX58 -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human RIG-I/DDX58 aa 900 to the C-terminus (C terminal). (NP_055129.2).
Database link: O95786 -
Positive control
- IHC-P: Human spleen, placenta and cortex tissues. ICC/IF: A431 and HeLa cells. Flow cyt: U937 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody (ab238254)
Paraformaldehyde-fixed, 0.15% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling RIG-I/DDX58 with ab238254 at 10 μg/ml in ICC/IF, followed by AlexaFluor® 488 conjugated secondary antibody at 2 μg/ml (green). The nuclear counter stain is DAPI (blue).
-ve control: Unimmunized Goat IgG at 10 μg/ml, followed by AlexaFluor® 488 conjugated secondary antibody at 2 μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIG-I/DDX58 antibody (ab238254)Paraffin-embedded human spleen tissue stained for RIG-I/DDX58 using ab238254 at 3.75 μg/ml in immunohistochemical analysis. AP staining.
-
Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody (ab238254)Paraformaldehyde-fixed, 0.15% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RIG-I/DDX58 with ab238254 at 10 μg/ml in ICC/IF, followed by AlexaFluor® 488 conjugated secondary antibody at 2 μg/ml (green). The nuclear counter stain is DAPI (blue).
-ve control: Unimmunized Goat IgG at 10 μg/ml, followed by AlexaFluor® 488 conjugated secondary antibody at 2 μg/ml.
-
Flow Cytometry - Anti-RIG-I/DDX58 antibody (ab238254)Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-X-100 permeabilized U937 (human histiocytic lymphoma cell line) cells labeling RIG-I/DDX58 with ab238254 at 10 μg/ml (blue) compared with unimmunized Goat IgG (black). An AlexaFluor®488 secondary antibody was used at 2 μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIG-I/DDX58 antibody (ab238254)Paraffin-embedded human placenta tissue stained for RIG-I/DDX58 using ab238254 at 3.75 μg/ml in immunohistochemical analysis. AP staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIG-I/DDX58 antibody (ab238254)Paraffin-embedded human cortex tissue stained for RIG-I/DDX58 using ab238254 at 3.75 μg/ml in immunohistochemical analysis. AP staining.
