Human Renin ELISA Kit (ab246545)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Renin ELISA Kit
See all Renin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.5% Inter-assay Sample n Mean SD CV% Serum 3 4.3% -
Sample type
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 105 104% - 106% Serum 112 109% - 117% Hep Plasma 103 99% - 108% EDTA Plasma 98 95% - 100% Cit plasma 105 102% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Renin ELISA Kit (ab246545) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Renin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Renin with 5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Renin Capture Antibody 1 x 600µl 10X Human Renin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Human Renin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
Involvement in disease
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
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Alternative names
- Angiotensin forming enzyme
- Angiotensin forming enzyme precursor
- Angiotensinogenase
see all -
Database links
- Entrez Gene: 5972 Human
- Omim: 179820 Human
- SwissProt: P00797 Human
- Unigene: 3210 Human
Images
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Other - Human Renin ELISA Kit (ab246545)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Renin Standard Curve ComparisonStandard Curve comparison between human Renin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Renin standard curve in Sample Diluent 50BS.The Renin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Renin in human serum and plasma samples.The concentrations of Renin were measured in duplicates, interpolated from the Renin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 75%, plasma (EDTA) 75%, plasma (citrate) 75%, and plasma (heparin) 75%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Renin concentration was determined to be 528 pg/mL in serum, 482 pg/mL in plasma (EDTA), 430 pg/mL in plasma (citrate)and 460 pg/mL in plasma (heparin).
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Interpolated concentrations of native Renin in human urine samples.The concentrations of Renin were measured in duplicates, interpolated from the Renin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 37.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Renin concentration was determined to be 2,514 pg/mL in urine.
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Serum from eight individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Renin concentration in neat serum was determined to be 796 pg/mL with a range of 126 – 1,757 pg/mL.
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Sandwich ELISA - Human Renin ELISA Kit (ab246545)To learn more about the advantages of recombinant antibodies see here.
