Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-8 antibody [E6] (ab32125)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E6] to Caspase-8
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-8 antibody [E6]
    See all Caspase-8 primary antibodies

  • Description

    Rabbit monoclonal [E6] to Caspase-8

  • Host species

    Rabbit

  • Specificity

    ab32125 should recognize all splice isoforms of Caspase-8.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Caspase-8 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q14790

  • Epitope

    ab32125 reacts with an epitope located in the N terminal region of caspase-8.

  • Positive control

    • WB: HeLa, SH-SY5Y, Jurkat (ab7899) and HAP1 whole cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-8 antibody [E6] (ab32125)
    Western blot - Anti-Caspase-8 antibody [E6] (ab32125)
    All lanes : Anti-Caspase-8 antibody [E6] (ab32125) at 1/3000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CASP8 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Lanes 1- 4: Merged signal (red and green). Green - ab32125 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab32125 was shown to react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type HeLa and CASP8 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 3000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Caspase-8 antibody [E6] (ab32125)
    Western blot - Anti-Caspase-8 antibody [E6] (ab32125)
    All lanes : Anti-Caspase-8 antibody [E6] (ab32125)

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : CASP8 knockout HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 55 kDa



    Lanes 1 and 2: Merged signal (red and green). Green - ab32125 observed at 55 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab32125 was shown to recognize Caspase-8 when Caspase-8 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. ab32125 and ab8226 (loading control to beta actin) were diluted 1/3000 and 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Caspase-8 antibody [E6] (ab32125)
    Western blot - Anti-Caspase-8 antibody [E6] (ab32125)
    Anti-Caspase-8 antibody [E6] (ab32125) at 1/3000 dilution + Jurkat cell lysate

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Anti-Caspase-8 antibody [E6] (ab32125)
    Anti-Caspase-8 antibody [E6] (ab32125)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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