Aldolase Activity Assay Kit (Colorimetric) (ab196994)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 100 µU
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Aldolase Assay Buffer 1 x 25ml Aldolase Developer 1 vial Aldolase Enzyme Mix 1 vial Aldolase Substrate 1 vial NADH Standard Yellow 1 vial Positive Control 1 vial -
Research areas
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Function
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. -
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
Involvement in disease
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis. -
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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Alternative names
- ALDA
- Aldo1
- ALDOA
see all
Images
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Example data
Typical NADH Standard Curve.
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Example dataAldolase activity in rat kidney and muscle, HeLa and 3T3 cells. Data obtained performing assay as described in assay protocol.
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Example dataRelative aldolase activity in lysates prepared from rat kidney (14.2 μg), rat muscle (10.64 μg), 3T3 cells (9.97 μg) and HeLa cells (11.35 μg).
