Anti-SCAI antibody [EPR4128] (ab124688)
![Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-241117-anti-scai-antibody-epr4128-immunofluorescence.jpg "Anti-SCAI antibody [EPR4128] (ab124688)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4128] to SCAI
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SCAI antibody [EPR4128]
See all SCAI primary antibodies -
Description
Rabbit monoclonal [EPR4128] to SCAI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human thymus, thyroid, and 293T cell lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4128 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (ab124688)
Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
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![Western blot - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-241112-anti-scai-antibody-epr4128-western-blot.jpg)
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-241110-anti-scai-antibody-epr4128-western-blot.jpg)
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified) + Human thyroid tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-241115-anti-scai-antibody-epr4128-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (ab124688)Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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![Flow Cytometry - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-241118-anti-scai-antibody-epr4128-flow-cytometry.jpg)
Flow Cytometry - Anti-SCAI antibody [EPR4128] (ab124688)Overlay histogram showing SH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in 160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
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![Western blot - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-138450-anti-scai-antibody-epr4128-western-blot.jpg)
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1000 dilution (unpurified)
Lane 1 : Human Thymus lysate
Lane 2 : Human Thyroid lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 70 kDa
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![Western blot - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-241111-anti-scai-antibody-epr4128-western-blot.jpg)
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/200 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-241114-anti-scai-antibody-epr4128-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (ab124688)Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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![Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-241116-anti-scai-antibody-epr4128-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (ab124688)Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
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![Flow Cytometry - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-138449-anti-scai-antibody-epr4128-flow-cytometry.jpg)
Flow Cytometry - Anti-SCAI antibody [EPR4128] (ab124688)Unpurified ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by Flow cytometry (red) compared to a nonspecific negative control antibody (green).
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![OI-RD Scanning - Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-138448-anti-scai-antibody-epr4128.jpg)
OI-RD Scanning - Anti-SCAI antibody [EPR4128] (ab124688) -
![Anti-SCAI antibody [EPR4128] (ab124688)](https://www.abcam.com/ps/products/124/ab124688/Images/ab124688-12-benefits-of-recombinant-antibodies.png)
Anti-SCAI antibody [EPR4128] (ab124688)
