Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIP-1 antibody [EPR7900(2)] (ab155992)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7900(2)] to TIP-1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TIP-1 antibody [EPR7900(2)]
    See all TIP-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7900(2)] to TIP-1

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TIP-1 aa 1-100. The exact sequence is proprietary.
    Database link: O14907

  • Positive control

    • WB: Mouse lung and heart tissue lysates. Rat lung and heart tissue lysate. NIH/3T3, A673, LLC1, and C6 whole cell lysates; IHC-P: Human thyroid carcinoma tissue; Flow-cyt: A673 cells.

  • General notes

    Previously labelled as TAX1BP3. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TIP-1 with purified ab155992 at 1/1000 dilution (2.05 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)
    Western blot - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)
    All lanes : Anti-TIP-1 antibody [EPR7900(2)] (ab155992) at 1/1000 dilution (Purified)

    Lane 1 : A-673 (Human muscle Ewing's Sarcoma) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 3 : LLC1 (Mus musculus lung rounded-loosely attached or floating) whole cell lysate
    Lane 4 : Mouse lung lysate
    Lane 5 : Mouse heart lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 7 : Rat lung lysate
    Lane 8 : Rat heart tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)
    Flow Cytometry - Anti-TIP-1 antibody [EPR7900(2)] (ab155992)

    Flow Cytometry analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling TIP-1 with purified ab155992 at 1/200 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-TIP-1 antibody [EPR7900(2)] (ab155992)
    Anti-TIP-1 antibody [EPR7900(2)] (ab155992)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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