Anti-RASA1 antibody [EP536Y] (ab40677)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP536Y] to RASA1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-RASA1 antibody [EP536Y]
See all RASA1 primary antibodies -
Description
Rabbit monoclonal [EP536Y] to RASA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat -
Immunogen
Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
Database link: P20936 -
Positive control
- WB: Rat brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP536Y -
Isotype
IgG -
Research areas
Images
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Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution + Rat brain lysate at 10 µg
Predicted band size: 96 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cleavage fragment)
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Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1:50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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