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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators

Anti-RASA1 antibody [EP536Y] (ab40677)

Price and availability

298 185 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-RASA1 antibody [EP536Y] (ab40677)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP536Y] to RASA1
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-RASA1 antibody [EP536Y]
    See all RASA1 primary antibodies
  • Description

    Rabbit monoclonal [EP536Y] to RASA1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
    Database link: P20936

  • Positive control

    • WB: Rat brain tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP536Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Images

  • Western blot - Anti-RASA1 antibody [EP536Y] (ab40677)
    Western blot - Anti-RASA1 antibody [EP536Y] (ab40677)
    Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution + Rat brain lysate at 10 µg

    Predicted band size: 96 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 55 kDa (possible cleavage fragment)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] (ab40677)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] (ab40677)

    Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1:50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-RASA1 antibody [EP536Y] (ab40677)
    Anti-RASA1 antibody [EP536Y] (ab40677)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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