Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-RASA1 antibody [EP536Y] (ab204006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP536Y] to RASA1
  • Suitable for: WB
  • Reacts with: Rat
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-RASA1 antibody [EP536Y]
    See all RASA1 primary antibodies

  • Description

    HRP Rabbit monoclonal [EP536Y] to RASA1

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
    Database link: P20936

  • Positive control

    • WB: Rat brain tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP536Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - HRP Anti-RASA1 antibody [EP536Y] (ab204006)
    Western blot - HRP Anti-RASA1 antibody [EP536Y] (ab204006)
    HRP Anti-RASA1 antibody [EP536Y] (ab204006) at 1/5000 dilution + Brain (Rat) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 116 kDa
    Observed band size: 140,45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204006 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-RASA1 antibody [EP536Y] (ab204006)
    HRP Anti-RASA1 antibody [EP536Y] (ab204006)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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