Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GNAI1 antibody [EPR9441(B)] (ab140125)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9441(B)] to GNAI1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-GNAI1 antibody [EPR9441(B)]
    See all GNAI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9441(B)] to GNAI1

  • Host species

    Rabbit

  • Specificity

    The immunogenic sequence of ab140125 has 92.9% homology with GNAI3 and 64% (4 continuous residues) homology with GNAI2 which might cause cross reactivity with GNAI3 and GNAI2.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GNAI1 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • SH-SY5Y, U-87 MG, Human glioma, and Human fetal brain lysates; Human endometrial carcinoma and thyroid carcinoma tissues.

  • General notes

     This product was previously labelled as G protein alpha inhibitor 1, G Protein alpha Inhibitor 1+2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
    Western blot - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
    All lanes : Anti-GNAI1 antibody [EPR9441(B)] (ab140125) at 1/1000 dilution

    Lane 1 : SH-SY5Y lysate
    Lane 2 : U-87 MG lysate
    Lane 3 : Human glioma lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 40 kDa

  • Flow Cytometry - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
    Flow Cytometry - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
    Overlay histogram showing SHSY-5Y cells stained with ab140125 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140125, 1/9999 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling GNAI1 with ab140125 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 antibody [EPR9441(B)] (ab140125)

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling GNAI1 with ab140125 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
    Anti-GNAI1 antibody [EPR9441(B)] (ab140125)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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