Recombinant human AMPK alpha 1 + AMPK beta 2 + AMPK gamma 3 protein (Active) (ab184885)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Tags: His tag C-Terminus, proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
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Product name
Recombinant human AMPK alpha 1 + AMPK beta 2 + AMPK gamma 3 protein (Active) -
Biological activity
The specific activity of ab184885 was determined to be 400 nmol/min/mg
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Purity
> 80 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
His tag C-Terminus , proprietary tag N-Terminus -
Additional sequence information
Recombinant full length AMPK alpha 1 (a.a's 1-559 of UniProt ID: Q13131) + AMPK beta 2 (a.a's 1-272 of UniProt ID: O43741) + AMPK gamma 3 (a.a.'s 1- 489 of UniProt ID:Q9UGI9). MWt 92, 62, and 108 KDa.
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Preparation and Storage
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Applications
Functional Studies
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
Q13131
MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQO43741
MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPIQ9UGI9
MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTSSSERIRGKRRAKALRWTRQKSVEEGEPPGQGEGPRSRPAAESTGLEATFPKTTPLAQADPAGVGTPPTGWDCLPSDCTASAAGSSTDDVELATEFPATEAWECELEGLLEERPALCLSPQAPFPKLGWDDELRKPGAQIYMRFMQEHTCYDAMATSSKLVIFDTMLEIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEQHKIETWREIYLQGCFKPLVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQDLGIGTFRDLAVVLETAPILTALDIFVDRRVSALPVVNECGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLS PAGIDALGAab204881 (Acetyl Coenzyme A Carboxylase peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Images
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Functional Studies - Recombinant human AMPK alpha 1 + AMPK beta 2 + AMPK gamma 3 protein (Active) (ab184885)
Kinase Assay demonstrating specific activity of ab184885.
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SDS-PAGE - Recombinant human AMPK alpha 1 + AMPK beta 2 + AMPK gamma 3 protein (Active) (ab184885)SDS-PAGE showing ab184885, approx MW ~92 kDa (A1), ~62 kDa (B2) and ~108 kDa (G3).