Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3667] to Lysophospholipase 1/LPL-I
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Lysophospholipase 1/LPL-I antibody [EPR3667]
See all Lysophospholipase 1/LPL-I primary antibodies
Description

Rabbit monoclonal [EPR3667] to Lysophospholipase 1/LPL-I
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Lysophospholipase 1/LPL-I aa 150-250. The exact sequence is proprietary.
Positive control
- WB: HAP1 and HEK-293T cell lysate; fetal liver, fetal heart, Human spleen, Human liver, Mouse heart, Mouse kidney, Mouse spleen Mouse liver, Rat liver and Rat kidney lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3667
Isotype

IgG
Research areas

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : LYPLA1 knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 25 kDa
Observed band size: 25 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab91606 was shown to react with Lysophospholipase 1/LPL-I in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266195 (knockout cell lysate ab257509) was used. Wild-type HEK-293T and LYPLA1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab91606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab91606 was shown to specifically react with LYPLA1 in wild-type HAP1 cells. No band was obsereved when LYPLA1 knockout samples were examined. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91606 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution (Purified)

Lane 1 : Human liver lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Mouse liver lysates
Lane 4 : Rat liver lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

Lane 1 : Fetal liver lysate
Lane 2 : Fetal heart lysate
Lane 3 : Human spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 25 kDa
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 25 kDa
Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

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