Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)
![Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)](https://www.abcam.com/ps/products/91/ab91606/Images/ab91606-447810-anti-lysophospholipase-1lpl-i-antibody-epr3667-western-blot-hek-293t-lysates.jpg "Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3667] to Lysophospholipase 1/LPL-I
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Lysophospholipase 1/LPL-I antibody [EPR3667]
See all Lysophospholipase 1/LPL-I primary antibodies -
Description
Rabbit monoclonal [EPR3667] to Lysophospholipase 1/LPL-I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide within Lysophospholipase 1/LPL-I aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: HAP1 and HEK-293T cell lysate; fetal liver, fetal heart, Human spleen, Human liver, Mouse heart, Mouse kidney, Mouse spleen Mouse liver, Rat liver and Rat kidney lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3667 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : LYPLA1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDaLanes 1- 2: Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab91606 was shown to react with Lysophospholipase 1/LPL-I in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266195 (knockout cell lysate ab257509) was used. Wild-type HEK-293T and LYPLA1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab91606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)](https://www.abcam.com/ps/products/91/ab91606/Images/ab91606-273883-ab91606KOWB.jpg)
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.ab91606 was shown to specifically react with LYPLA1 in wild-type HAP1 cells. No band was obsereved when LYPLA1 knockout samples were examined. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91606 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)](https://www.abcam.com/ps/products/91/ab91606/Images/ab91606-339716-ab91606WB1.jpg)
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution (Purified)
Lane 1 : Human liver lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Mouse liver lysates
Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
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![Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)](https://www.abcam.com/ps/products/91/ab91606/Images/Lysophospholipase-1-Primary-antibodies-ab91606-1.JPG)
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : Fetal heart lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 25 kDa
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![Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)](https://www.abcam.com/ps/products/91/ab91606/Images/Lysophospholipase-1-Primary-antibodies-ab91606-2.JPG)
Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 25 kDa
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![Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)](https://www.abcam.com/ps/products/91/ab91606/Images/ab91606-6-benefits-of-recombinant-antibodies.png)
Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)
