Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3666] to Lysophospholipase 1/LPL-I
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Lysophospholipase 1/LPL-I antibody [EPR3666]
    See all Lysophospholipase 1/LPL-I primary antibodies

  • Description

    Rabbit monoclonal [EPR3666] to Lysophospholipase 1/LPL-I

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Lysophospholipase 1/LPL-I aa 50-150.

  • Positive control

    • Human fetal liver, Human fetal heart and Human spleen cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3666

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600)
    Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600)

    Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
    Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
    Lane 3: Mouse spleen whole cell lysate (40 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab91600 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab91600 was shown to specifically react with LYPLA1 in wild-type HAP1 cells. No band was observed when LYPLA1 knockout samples were examined. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91600 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with ab216773, Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed and ab216776, Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600)
    Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600)
    All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 25 kDa

  • Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600)
    Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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