Protease and Phosphatase Inhibitor Cocktail (EDTA-free) (ab201120)
Overview
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Product name
Protease and Phosphatase Inhibitor Cocktail (EDTA-free) -
Product overview
To protect against both protease and phosphatase in one solution try our dual cocktail (also available with EDTA).
AEBSF free and mass spectroscopy compatible – water soluble.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases
Target phosphatases - Serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases
Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate
Form: Supplied in water (1 ml)
Preparation: Recommended dilution is 1:9. They are provided in a 10x formulation and a 1X solution is a good starting point. The working concentration should be optimized for each individual application.
Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.
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Notes
Individual components and concentrations:
Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
β-Glycerophosphate: serine/threonine phosphatase inhibitor (10 mM)
Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (50 mM)
Sodium orthovanadate: protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
Sodium pyrophosphate decahydrate: serine/threonine phosphatase inhibitor (10 mM)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 ml Protease and Phosphatase Inhibitor Cocktail (EDTA-free) 1 x 1ml
