Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3667] to Lysophospholipase 1/LPL-I - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free
    See all Lysophospholipase 1/LPL-I primary antibodies

  • Description

    Rabbit monoclonal [EPR3667] to Lysophospholipase 1/LPL-I - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, HAP1, Human liver and fetal heart, liver and spleen tissue lysates; Mouse liver, heart, spleen and kidney tissue lysate; Rat liver and kidney tissue lysate.

  • General notes

    Ab239901 is the carrier-free version of ab91606. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239901 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3667

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : LYPLA1 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab91606).

    Lanes 1- 2: Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab91606 was shown to react with Lysophospholipase 1/LPL-I in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266195 (knockout cell lysate ab257509) was used. Wild-type HEK-293T and LYPLA1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab91606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)

    This data was developed using ab91606, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (40 µg)

    Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab91606 was shown to specifically react with LYPLA1 in wild-type HAP1 cells. No band was obsereved when LYPLA1 knockout samples were examined. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91606 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution (Purified)

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using ab91606, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

    Lane 1 : Fetal liver tissue lysate
    Lane 2 : Fetal heart tissue lysate
    Lane 3 : Human spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 25 kDa



    This data was developed using ab91606, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 25 kDa



    This data was developed using ab91606, the same antibody clone in a different buffer formulation.

  • Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)
    Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] - BSA and Azide free (ab239901)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.