Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10338(2)] to HDAC8 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free
    See all HDAC8 primary antibodies

  • Description

    Rabbit monoclonal [EPR10338(2)] to HDAC8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, K562 and HeLa whole cell lysates.

  • General notes

    ab232643 is the carrier-free version of ab187139.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
    Western blot - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HDAC8 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate (20 µg)
    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab187139. Red - loading control, ab7291, observed at 52 kDa.
    ab187139 was shown to specifically react with HDAC8 when HDAC8 knockout samples were used. Wild-type and HDAC8 knockout samples were subjected to SDS-PAGE. ab187139 and ab7291 (loading control to alpha tubulin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187139).

  • Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
    Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

    Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human fetal kidney tissue lysate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187139).

  • Flow Cytometry - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
    Flow Cytometry - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

    Flow cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187139).

  • Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
    Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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