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Anti-RASA1 antibody [B4F8] (ab2922)

Key features and details

  • Mouse monoclonal [B4F8] to RASA1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-RASA1 antibody [B4F8]
    See all RASA1 primary antibodies

  • Description

    Mouse monoclonal [B4F8] to RASA1

  • Host species

    Mouse

  • Specificity

    This antibody is specific for RASA1 (an alternate name of GAP).

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Non human primates

  • Immunogen

    Recombinant full length protein corresponding to Human RASA1.

  • Epitope

    Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3.

  • Positive control

    • RS-2 cell lysate. Mouse fibroblast cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • Purity

    Protein A purified

  • Primary antibody notes

    GTPase Activating Protein (GAP) is capable of stimulating the hydrolysis of GTP by ras p21 proteins, though GAP has little effect on the oncogenic forms of ras. It is also known that several tyrosine kinases such as platelet derived growth factor receptor and epidermal growth factor receptor are involved in the tyrosine phosphorylation of GAP. It has therefore been suggested that GAP may provide a connection or link between growth factor receptors and the ras p21 family.

  • Clonality

    Monoclonal

  • Clone number

    B4F8

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
    Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

    ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
    Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

    ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
    Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

    ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Western blot - Anti-RASA1 antibody [B4F8] (ab2922)
    Western blot - Anti-RASA1 antibody [B4F8] (ab2922)
    All lanes : Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Mouse brain cell lysate

    Lysates/proteins at 25 µg per lane.

    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

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