Glucose Dehydrogenase Assay Kit (Colorimetric) (ab102532)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell culture media, Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mU/well
Overview
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Product name
Glucose Dehydrogenase Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media -
Assay type
Enzyme activity -
Sensitivity
> 0.01 mU/well -
Assay time
2h 00m -
Product overview
Abcam's Glucose Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (λ = 450 nm).
Visit our FAQs page for tips and troubleshooting. -
Notes
This assay detects GDH activity as low as 0.01 mU.
Developer (Lyophilized) is light sensitive and should be protected from light during storage and preparation.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Developer (Lyophilized) Red 1 vial GDH Assay Buffer NM 1 x 25ml GDH Positive Control (Lyophilized) Green 1 vial Glucose Blue 1 x 1ml NADH Standard (Lyophilized) Yellow 1 vial -
Research areas
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Relevance
Glucose dehydrogenase (NAD+) (EC 1.1.1.118) is an enzyme that catalyzes the chemical reaction: D-glucose + NAD+ = D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. -
Alternative names
- GDH
Images
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Functional Studies - Glucose Dehydrogenase Activity Detection Kit (ab102532)Example of Bovine Liver Sample test curves obtained using ab102532
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Functional Studies - Glucose Dehydrogenase Activity Detection Kit (ab102532)Example of NADH standard curve obtained using ab102532