Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PFKL antibody [EPR11904] (ab181064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11904] to PFKL
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PFKL antibody [EPR11904]
    See all PFKL primary antibodies

  • Description

    Rabbit monoclonal [EPR11904] to PFKL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, A431, HeLa, C6, Raw264.7, PC-12 and NIH-3T3 cell lysates; HeLa and C6 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PFKL antibody [EPR11904] (ab181064)
    Western blot - Anti-PFKL antibody [EPR11904] (ab181064)
    All lanes : Anti-PFKL antibody [EPR11904] (ab181064) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 85 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-PFKL antibody [EPR11904] (ab181064)
    Western blot - Anti-PFKL antibody [EPR11904] (ab181064)
    All lanes : Anti-PFKL antibody [EPR11904] (ab181064) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution

    Predicted band size: 85 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody [EPR11904] (ab181064)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody [EPR11904] (ab181064)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) staining PFKL with ab181064 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Flow Cytometry - Anti-PFKL antibody [EPR11904] (ab181064)
    Flow Cytometry - Anti-PFKL antibody [EPR11904] (ab181064)

    Flow cytometric analysis of 2% paraformaldehyde-fixed C6 cells labeling PFKL with ab181064 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Anti-PFKL antibody [EPR11904] (ab181064)
    Anti-PFKL antibody [EPR11904] (ab181064)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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