Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23334-171] to Resistin - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Resistin antibody [EPR23334-171] - BSA and Azide free
    See all Resistin primary antibodies

  • Description

    Rabbit monoclonal [EPR23334-171] to Resistin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U937 whole cell lysate; Human white fat tissue lysate; Recombinant human Resistin protein. Flow Cyt: U937 cells. IP: U937 whole cell lysate.

  • General notes

    ab275887 is the carrier-free version of ab275878. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275887 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    Western blot - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    All lanes : Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution

    Lane 1 : U937 (human histiocytic lymphoma monocyte) whole cell lysate
    Lane 2 : THP-1 (human monocytic leukemia monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    This data was developed using ab275878, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:16532325, 14550293).

    Low expression: THP-1 (PMID:16532325 ).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Flow Cytometry - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    Flow Cytometry - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)

    This data was developed using ab275878, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U937 (human histiocytic lymphoma monocyte) cells labelling Resistin with ab275878 at 1/500 dilution (0.1µg)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    Immunoprecipitation - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)

    This data was developed using ab275878, the same antibody clone in a different buffer formulation.

    Resistin was immunoprecipitated from 0.35 mg U937 (human histiocytic lymphoma monocyte) whole cell lysate with ab275878 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275878 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: U937 whole cell lysate 10 µg

    Lane 2: ab275878 IP in U937 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275878 in U937 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds.

     

  • Western blot - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    Western blot - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution + Human white fat tissue lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    This data was developed using ab275878, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Western blot - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    Western blot - Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution + Recombinant human Resistin protein, 20 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 11 kDa



    This data was developed using ab275878, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

  • Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)
    Anti-Resistin antibody [EPR23334-171] - BSA and Azide free (ab275887)

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