Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13509(B)] to hnRNP M1-M4
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-hnRNP M1-M4 antibody [EPR13509(B)]
    See all hnRNP M1-M4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13509(B)] to hnRNP M1-M4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human hnRNP M1-M4 aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: P52272

  • Positive control

    • WB: HeLa, HepG2, Jurkat, 293T, Mouse brain, and Rat brain lysates. IHC-P: human kidney, human kidney carcinoma, mouse testis, and rat cerebrum tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling hnRNP M1-M4 with Purified ab177957 at 1:4000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Western blot - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    All lanes : Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa
    Observed band size: 78 kDa

  • Flow Cytometry - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Flow Cytometry - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling hnRNP M1-M4 with Purified ab177957 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling hnRNP M1-M4 with Purified ab177957 at 1:100 dilution (9.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling hnRNP M1-M4 with Purified ab177957 at 1:4000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling hnRNP M1-M4 with Purified ab177957 at 1:4000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Western blot - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    All lanes : Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa
    Observed band size: 78 kDa


    Exposure time: 3 minutes


    Blocking and Diluting buffer 5% NFDM/TBST

  • Western blot - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Western blot - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    All lanes : Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 78 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)

    Immunohistochemical analysis of Human kidney carcinoma tissue labeling hnRNP M1-M4 using ab177957 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)

    Immunofluorescence analysis of HeLa cells labeling hnRNP M1-M4 using ab177957 at 1/100 dilution.

  • Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)
    Anti-hnRNP M1-M4 antibody [EPR13509(B)] (ab177957)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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