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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing

Anti-hnRNP M1-M4 antibody [HL374] (ab255958)

Price and availability

307 566 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [HL374] to hnRNP M1-M4
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-hnRNP M1-M4 antibody [HL374]
    See all hnRNP M1-M4 primary antibodies
  • Description

    Mouse monoclonal [HL374] to hnRNP M1-M4
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and MCF7 whole cell lysate. ICC/IF: HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    HL374
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling hnRNP M1-M4 with ab255958 at 9.26µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

  • Western blot - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
    Western blot - Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
    All lanes : Anti-hnRNP M1-M4 antibody [HL374] (ab255958) at 0.463 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 78 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 sec; Lane 2: 3 secs.

  • Anti-hnRNP M1-M4 antibody [HL374] (ab255958)
    Anti-hnRNP M1-M4 antibody [HL374] (ab255958)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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