Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VPS26 antibody [EPR13456] (ab181352)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13456] to VPS26
  • Suitable for: WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-VPS26 antibody [EPR13456]
    See all VPS26 primary antibodies

  • Description

    Rabbit monoclonal [EPR13456] to VPS26

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS26 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O75436

  • Positive control

    • HepG2, HeLa, 293T, and A431 cell lysates; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13456

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-VPS26 antibody [EPR13456] (ab181352)
    Western blot - Anti-VPS26 antibody [EPR13456] (ab181352)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181352 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab181352 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab181352 and ab18058 (loading control to Vinculin) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [EPR13456] (ab181352)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [EPR13456] (ab181352)

    Immunofluorescent analysis of HeLa cells labeling VPS26 with ab181352 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Western blot - Anti-VPS26 antibody [EPR13456] (ab181352)
    Western blot - Anti-VPS26 antibody [EPR13456] (ab181352)
    All lanes : Anti-VPS26 antibody [EPR13456] (ab181352) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 38 kDa

  • Immunoprecipitation - Anti-VPS26 antibody [EPR13456] (ab181352)
    Immunoprecipitation - Anti-VPS26 antibody [EPR13456] (ab181352)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate pulling down VPS26 using ab181352 at 1/10 dilution.

  • Anti-VPS26 antibody [EPR13456] (ab181352)
    Anti-VPS26 antibody [EPR13456] (ab181352)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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