Anti-BMX (phospho Y566) antibody (ab59409)
Key features and details
- Rabbit polyclonal to BMX (phospho Y566)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BMX (phospho Y566) antibody
See all BMX primary antibodies -
Description
Rabbit polyclonal to BMX (phospho Y566) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of ETK only when phosphorylated at tyrosine 566. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).
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Positive control
- Human thyroid gland tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMX (phospho Y566) antibody (ab59409)Immunohistochemical analysis of paraffin embedded human thyroid gland tissue using ab59409 at a 1/50 dilution, in the presence (right) and absence (left) of immunizing phosphopeptide.
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Western blot - Anti-BMX (phospho Y566) antibody (ab59409)Anti-BMX (phospho Y566) antibody (ab59409) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 78 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
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Western blot - Anti-BMX (phospho Y566) antibody (ab59409)All lanes : Anti-BMX (phospho Y566) antibody (ab59409)
Lane 1 : Extracts from HeLa cells treated with Serum 20% 15'
Lane 2 : Extracts from HeLa cells treated with Serum 20% 15' and with the synthesized peptide.
Predicted band size: 78 kDa
