Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19235] to SAA1 + SAA2 - BSA and Azide free
  • Suitable for: WB, IP, IHC-Fr, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free
    See all SAA1 + SAA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19235] to SAA1 + SAA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251264 is the carrier-free version of ab199030. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251264 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR19235

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)

    This data was developed using ab199030, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse liver is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)

    This data was developed using ab199030, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Negative control: mouse kidney (PMID:19747761; PMID: 15623807). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    This data was developed using ab199030, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/100 dilution (green). Cytoplasmic staining on mouse liver is observed. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    All lanes : Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/10000 dilution

    Lane 1 : Recombinant mouse SAA1 protein (aa20-122) at 0.01 µg
    Lane 2 : GST and His-tagged recombinant mouse SAA2 protein (aa20-122) at 0.005 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa


    Exposure time: 2 seconds


    This data was developed using ab199030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/2000 dilution + GST and His-tagged recombinant mouse SAA3 protein (aa1-122) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa


    Exposure time: 3 minutes


    This data was developed using ab199030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    All lanes : Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/1000 dilution

    Lane 1 : Mouse plasma
    Lane 2 : Mouse serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa



    This data was developed using ab199030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 30 seconds.

  • Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    This data was developed using ab199030, the same antibody clone in a different buffer formulation.SAA1 + SAA2 was immunoprecipitated from 1 mg of mouse serum with ab199030 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199030 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse serum 10 μg (Input). Lane 2: ab199030 IP in mouse serum. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199030 in mouse serum. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.
  • Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)
    Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (ab251264)

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