Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB13 antibody [EPR14109(B)] (ab180936)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14109(B)] to RAB13
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-RAB13 antibody [EPR14109(B)]
    See all RAB13 primary antibodies

  • Description

    Rabbit monoclonal [EPR14109(B)] to RAB13

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and A431 cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RAB13 antibody [EPR14109(B)] (ab180936)
    Western blot - Anti-RAB13 antibody [EPR14109(B)] (ab180936)
    All lanes : Anti-RAB13 antibody [EPR14109(B)] (ab180936) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : RAB13 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : Hek293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 23 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab180936 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab180936 was shown to specifically react with RAB13 in wild-type HAP1 cells as signal was lost in RAB13 knockout cells. Wild-type and RAB13 knockout samples were subjected to SDS-PAGE. Ab180936 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-RAB13 antibody [EPR14109(B)] (ab180936)
    Western blot - Anti-RAB13 antibody [EPR14109(B)] (ab180936)
    All lanes : Anti-RAB13 antibody [EPR14109(B)] (ab180936) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    Lane 2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 10 µg

    Predicted band size: 23 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-RAB13 antibody [EPR14109(B)] (ab180936)
    Immunoprecipitation - Anti-RAB13 antibody [EPR14109(B)] (ab180936)

    Lysate from HeLa cells was immunoprecipitated with ab180936 at a 1/70 dilution. For the subsequent blot, ab180936 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Second land is the negative control.

  • Anti-RAB13 antibody [EPR14109(B)] (ab180936)
    Anti-RAB13 antibody [EPR14109(B)] (ab180936)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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