Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [7G8A4] to RAB13
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-RAB13 antibody [7G8A4]
See all RAB13 primary antibodies
Description

Mouse monoclonal [7G8A4] to RAB13
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to RAB13 aa 50-200. Expressed in E.coli.
Database link: P51153

Run BLAST with Run BLAST with
Positive control
- WB: Recombinant human RAB13 (aa 66-200) protein; RAB13 (aa 66-200)-hIgG-Fc-transfected HEK-293 cell lysate. IHC-P: Human ovarian cancer and bladder cancer tissue. ICC/IF: HeLa cells. Flow cyt: K562 cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

7G8A4
Isotype

IgG1
Research areas

Western blot - Anti-RAB13 antibody [7G8A4] (ab233810)

Anti-RAB13 antibody [7G8A4] (ab233810) at 1/500 dilution + Recombinant human RAB13 (aa 66-200) protein

Predicted band size: 23 kDa

Expected MW is 41 kDa.
Western blot - Anti-RAB13 antibody [7G8A4] (ab233810)

All lanes : Anti-RAB13 antibody [7G8A4] (ab233810) at 1/500 dilution

Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : RAB13 (aa 66-200)-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 23 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RAB13 antibody [7G8A4] (ab233810)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RAB13 (green) using ab233810 at 1/200 dilution in ICC/IF. Blue: DRAQ5 DNA dye. Actin filaments are stained with AlexaFluor^®-555-Phalloidin (red).
Flow Cytometry - Anti-RAB13 antibody [7G8A4] (ab233810)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells labeling RAB13 with ab233810 at 1/200 dilution (green) compared with a negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB13 antibody [7G8A4] (ab233810)

Paraffin-embedded human ovarian cancer tissue stained for RAB13 using ab233810 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB13 antibody [7G8A4] (ab233810)

Paraffin-embedded human bladder cancer tissue stained for RAB13 using ab233810 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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