Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB13 antibody [7G8A4] (ab233810)

Key features and details

  • Mouse monoclonal [7G8A4] to RAB13
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-RAB13 antibody [7G8A4]
    See all RAB13 primary antibodies

  • Description

    Mouse monoclonal [7G8A4] to RAB13

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to RAB13 aa 50-200. Expressed in E.coli.
    Database link: P51153

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Recombinant human RAB13 (aa 66-200) protein; RAB13 (aa 66-200)-hIgG-Fc-transfected HEK-293 cell lysate. IHC-P: Human ovarian cancer and bladder cancer tissue. ICC/IF: HeLa cells. Flow cyt: K562 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-RAB13 antibody [7G8A4] (ab233810)
    Western blot - Anti-RAB13 antibody [7G8A4] (ab233810)
    Anti-RAB13 antibody [7G8A4] (ab233810) at 1/500 dilution + Recombinant human RAB13 (aa 66-200) protein

    Predicted band size: 23 kDa



    Expected MW is 41 kDa.

  • Western blot - Anti-RAB13 antibody [7G8A4] (ab233810)
    Western blot - Anti-RAB13 antibody [7G8A4] (ab233810)
    All lanes : Anti-RAB13 antibody [7G8A4] (ab233810) at 1/500 dilution

    Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : RAB13 (aa 66-200)-hIgG-Fc-transfected HEK-293 cell lysate

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB13 antibody [7G8A4] (ab233810)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB13 antibody [7G8A4] (ab233810)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RAB13 (green) using ab233810 at 1/200 dilution in ICC/IF. Blue: DRAQ5 DNA dye. Actin filaments are stained with AlexaFluor®-555-Phalloidin (red).

  • Flow Cytometry - Anti-RAB13 antibody [7G8A4] (ab233810)
    Flow Cytometry - Anti-RAB13 antibody [7G8A4] (ab233810)

    Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells labeling RAB13 with ab233810 at 1/200 dilution (green) compared with a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB13 antibody [7G8A4] (ab233810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB13 antibody [7G8A4] (ab233810)

    Paraffin-embedded human ovarian cancer tissue stained for RAB13 using ab233810 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB13 antibody [7G8A4] (ab233810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB13 antibody [7G8A4] (ab233810)

    Paraffin-embedded human bladder cancer tissue stained for RAB13 using ab233810 at 1/200 dilution in immunohistochemical analysis. DAB staining.

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