Anti-Vinculin antibody [VIN-54] (ab130007)
![Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.com/ps/products/130/ab130007/Images/ab130007-356277-anti-vinculin-antibody-vin-54-western-blot-human.JPG "Anti-Vinculin antibody [VIN-54] (ab130007)")
Key features and details
- Mouse monoclonal [VIN-54] to Vinculin
- Suitable for: ICC/IF, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Vinculin antibody [VIN-54]
See all Vinculin primary antibodies -
Description
Mouse monoclonal [VIN-54] to Vinculin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-Fr HumanWB MouseRatHumanAfrican green monkey
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Immunogen
Human Vinculin purified from uterus.
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Positive control
- WB: Hela, HepG2, Cos-7, U-87 MG, PC-12, RH35, HEPA1-6 and NIH-3T3 whole cell lysates; ICC/IF: Human mammary cancer cells; IHC-P: Human breast tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.001% Sodium azide
Constituents: 1.2% Sodium acetate, 0.2% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
VIN-54 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)All lanes : Anti-Vinculin antibody [VIN-54] (ab130007) at 0.5 µg/ml
Lane 1 : Hela whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : Monkey COS-7 whole cell lysates
Lane 4 : U-87MG whole cell lysates
Lane 5 : Rat PC-12 whole cell lysates
Lane 6 : Rat RH35 whole cell lysates
Lane 7 : Mouse HEPA1-6 whole cell lysates
Lane 8 : Mouse NIH3T3 whole cell lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti- mouse IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 124 kDa
Observed band size: 124 kDaThe signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT.
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![Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.com/ps/products/130/ab130007/Images/ab130007-356276-anti-vinculin-antibody-vin-54-immunohistochemistry-human-placenta.JPG)
Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)Immunohistochemical analysis of frozen sections of human placenta tissue labelling Vinculin with ab130007 at 1μg/ml, followed by a biotinylated goat anti-mouse IgG secondary antibody. The tissue was blocked with 10% goat serum. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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![Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.com/ps/products/130/ab130007/Images/ab130007-356278-anti-vinculin-antibody-vin-54-immunocytochemistry-immunofluorescence-mammary-gland-cancer-human.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [VIN-54] (ab130007)Immunocytochemistry/Immunofluorescent analysis of human mammary cancer cells labelling Vinculin with ab130007 at 2μg/ml, followed by a DyLight®488 Conjugated Goat Anti-Mouse secondary antibody at 1/100 dilution. DAPI was used as a nuclear counterstain.
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![Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.com/ps/products/130/ab130007/Images/ab130007-356275-anti-vinculin-antibody-vin-54-immunohistochemistry-placenta-human.JPG)
Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)Immunohistochemical analysis of frozen sections of human placenta tissue labelling Vinculin with ab130007 at 1μg/ml, followed by a biotinylated goat anti-mouse IgG secondary antibody. The tissue was blocked with 10% goat serum. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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![Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.com/ps/products/130/ab130007/Images/ab130007-286744-anti-vinculin-antibody-vin-54-western-blot.jpg)
Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)All lanes : Anti-Vinculin antibody [VIN-54] (ab130007) at 1/10000 dilution
Lane 1 : HAP1
Lane 2 : HEK293
Lane 3 : U-2 OS
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 124 kDaab130007 was shown to recognize vinculin when whole cell lysates were subjected to SDS-PAGE. The blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes which was incubated overnight at 4°C at 0.1 μg antibody per mL and developed with Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibody at 1/15000 dilution for 1 hour at room temperature before imaging.
