Anti-PLK1 antibody [AZ34] (ab12211)
![Anti-PLK1 antibody [AZ34] (ab12211)](https://www.abcam.com/ps/products/12/ab12211/Images/ab12211_1.jpg "Anti-PLK1 antibody [AZ34] (ab12211)")
Key features and details
- Mouse monoclonal [AZ34] to PLK1
- Suitable for: WB
- Reacts with: Human, Xenopus laevis
- Isotype: IgG2a
Overview
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Product name
Anti-PLK1 antibody [AZ34]
See all PLK1 primary antibodies -
Description
Mouse monoclonal [AZ34] to PLK1 -
Host species
Mouse -
Specificity
The cross-reactivity of this antibody regards the various isoforms of PLK is not known. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human, Xenopus laevis -
Immunogen
Recombinant full length protein (Xenopus laevis).
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Positive control
- Hela cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AZ34 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-PLK1 antibody [AZ34] (ab12211) This image is courtesy of Julian Gannon, CRUK
Western blot using ab12211.
Lane 1: Mitotic CSF Xenopus egg extract
Lane 2: Interphase Xenopus egg extract
Lane 3: HeLa cell extractNote that the phosphorylated form of Plx from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.
Western blot using ab12211.Lane 1: Mitotic CSF Xenopus egg extract
Lane 2: Interphase Xenopus egg extract
Lane 3: HeLa cell extract
Note that the phosphorylated form of Plx from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract. -
![Western blot - Anti-PLK1 antibody [AZ34] (ab12211)](https://www.abcam.com/ps/products/12/ab12211/Images/ab12211-19757-anti-plk1-antibody-az34-western-blot.jpg)
Western blot - Anti-PLK1 antibody [AZ34] (ab12211)Anti-PLK1 antibody [AZ34] (ab12211) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 68 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
